[Biofilms] Thank you for your information on biofilm questins but i have more questions

merhawi debesai via biofilms%40net.bio.net (by samiboy_4ever from yahoo.com)
Tue Mar 11 03:40:45 EST 2008


Thank you so much for your brief information on the previous biofilm questions. I also have another question.
   
  Q. usually, procedures followed in the lab for bacterial surface analysis are destructive to the bacterial surface composition which might ofcourse lead to wrong conclusion.
  -       Washing procedure
  -       Type of washing and resuspension medium used
                    -    Speed of centrifugation
  Thus, does any body have any idea on how to handle these procedures so that to minimize the damage?
   
  thank you so much agian.
   
  tesfalem 
  
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Today's Topics:

1. Re: Biofilm experiment questions (bob)


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Message: 1
Date: Tue, 04 Mar 2008 20:20:22 -0800
From: bob 
Subject: [Biofilms] Re: Biofilm experiment questions
To: bionet-microbiology-biofilms from magpie.bio.indiana.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

On Sun, 2 Mar 2008 05:09:08 -0800 (PST), merhawi debesai
wrote:

>Hi,
> Thank you so much for your information in advance.
> Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions:
> 

> 2. The second question is regarding the preparation of bacterial strains for 
> surface analysis. 
> i. Usually, we prepare the test strains in suspensions. However, it is 
> known that there....difference between planktonic and sessile (biofilm) 
> bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?!

I think you just answered your own question.

Not sure if they make a different EPS when sessile, rather than just
more of it. But seems to me you would want to at least check.

Once you get your methodology running, try comparing.


By the way, there is a nice overview article in February issue of
Microbe:

How Pseudomonas aeruginosa Regulates Surface Behaviors
George A. O’Toole
At surfaces, these bacteria either form biofilms or swarm, a
regulated behavior with
important consequences for pathogenesis
http://www.asm.org/microbe/index.asp?bid=55982

bob



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