[Biofilms] confocal microscopy related query

nidhi bhargava via biofilms%40net.bio.net (by nidhiatchess from gmail.com)
Sat Feb 20 08:20:09 EST 2010


Dear Sir

i am working on a project on quorum sensing and quenching of pathogenic
microbes.

Please help me with the protocol that i should follow to take a confocal
scan of the biofilm.
so far i have checked the effect of a plant exact on the biofilm formation
of pseudomonas. the treatment showed reduction in biofilm when quantified
with crystal violet staining and 33% GAA washing method. further i wish to
see the reduction effect by confocal microscopy. I donot wish to check the
dead live cell counts. so can you please suggest how should i prepare the
sample for confocal microscopy..can i use 35mm petri plate for growing the
biofilm.


hoping for a reply.
thanking you.

regards
nidhi bhargava
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