[Biofilms] Query - Nucleic acid extraction from biofilms

prem saran tirumalai via biofilms%40net.bio.net (by premtsaran from gmail.com)
Mon Jul 19 07:27:55 EST 2010


Hi,

1. What is the most appropriate method for growing biofilms if the objective
is to obtain atleast 2ug of DNA or RNA ?
2. I choose to grow them in static mode over a glass slide 1" x 3" immersed
in a culture broth, will this method work?
3. If this be the method after how many hours of incubation will i be able
to visualise the film layer ?
4. Alternatively can i use the method as shown in the following link for the
aforesaid objective.

http://www.biofilmbook.hypertextbookshop.com/public_version/contents/appendices/appendix001/pages/page013.html

I need some expert advice on the subject.

Thanks and regards,

Prem Saran
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://www.bio.net/bionet/mm/biofilms/attachments/20100719/1be88571/attachment.html


More information about the Biofilms mailing list