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<div> Thank you so much for your brief information on the previous biofilm questions. I also have another question.</div> <div> </div> <div>Q. usually, procedures followed in the lab for bacterial surface analysis are destructive to the bacterial surface composition which might ofcourse lead to wrong conclusion.</div> <div class=MsoNormal style="MARGIN: 0in 0in 0pt 1in; TEXT-INDENT: -0.25in; mso-list: l0 level2 lfo1; tab-stops: list 1.0in">-       Washing procedure</div> <div class=MsoNormal style="MARGIN: 0in 0in 0pt 1in; TEXT-INDENT: -0.25in; mso-list: l0 level2 lfo1; tab-stops: list 1.0in">-       Type of washing and resuspension medium used</div> <div>                  -    Speed of centrifugation</div> <div>Thus, does any body have any idea on how to handle these procedures so that to minimize the damage?</div> <div> </div> <div>thank you so much agian.</div> <div> </div> <div>tesfalem </div> <div> biofilms-request@oat.bio.indiana.edu wrote:</div> <BLOCKQUOTE class=replbq style="PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #1010ff 2px solid">Send Biofilms mailing list submissions to biofilms@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/biofilms or, via email, send a message with subject or body 'help' to biofilms-request@net.bio.net You can reach the person managing the list at biofilms-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Biofilms digest..." Today's Topics: 1. Re: Biofilm experiment questions (bob) ---------------------------------------------------------------------- Message: 1 Date: Tue, 04 Mar 2008 20:20:22 -0800 From: bob <BBX107@EXCITE.XXXX.COM> Subject: [Biofilms] Re: Biofilm experiment questions To: bionet-microbiology-biofilms@magpie.bio.indiana.edu Message-ID: <FP7SS3HDPO0VRKL9GC8D9DK3LA73OK7FTU@4AX.COM> Content-Type: text/plain; charset=ISO-8859-1 On Sun, 2 Mar 2008 05:09:08 -0800 (PST), merhawi debesai <SAMIBOY_4EVER@YAHOO.COM>wrote: >Hi, > Thank you so much for your information in advance. > Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions: > > 2. The second question is regarding the preparation of bacterial strains for > surface analysis. > i. Usually, we prepare the test strains in suspensions. However, it is > known that there....difference between planktonic and sessile (biofilm) > bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?! I think you just answered your own question. Not sure if they make a different EPS when sessile, rather than just more of it. But seems to me you would want to at least check. Once you get your methodology running, try comparing. By the way, there is a nice overview article in February issue of Microbe: How Pseudomonas aeruginosa Regulates Surface Behaviors George A. O’Toole At surfaces, these bacteria either form biofilms or swarm, a regulated behavior with important consequences for pathogenesis http://www.asm.org/microbe/index.asp?bid=55982 bob ------------------------------ _______________________________________________ Biofilms mailing list Biofilms@net.bio.net http://www.bio.net/biomail/listinfo/biofilms End of Biofilms Digest, Vol 16, Issue 2 *************************************** </BLOCKQUOTE> <hr size=1>Be a better friend, newshound, and know-it-all with Yahoo! Mobile. <a href="http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ "> Try it now.</a>

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