Tissue Culture Course

MYRNA E. WATANABE SCF7 at manvax.bitnet
Wed Apr 11 23:12:00 EST 1990


The Biotechnology Program of Manhattan College and the College of Mt.
St. Vincent is sponsoring:

A SHORT COURSE IN BASIC TISSUE CULTURE

JUNE 4-8, 1990   9:00 AM-5:00 PM

This short course in the culturing of mammalian cells is presented by
means of hands-on procedures and demonstrations.  All the essential
apsects and contemporary methods of growing cells in culture will be
covered.  There will be lectures on the cultured cell, monolayer and
suspension culture systems, contamination, contemporary methods of
cell identification, and production of monoclonal antibodies.  Hands-on
procedures will include pipetting, media preparation and filtration, basic phase
    contrast microscopy, the hemocytometer method of cell counting, cryogenic
preservation, culturing anchorage dependent cells, primary cell
culturing, cell identification using immunohistofluorescence,
karyotyping, and mycoplasma detection.  Demonstrations on the use of
cryogenic equipment, various types of culture vessels, DNA
characterization, two dimensional gel electrophoresis of cultured cell
proteins emphasizing labeling, isoelectric focusing, polyacrylamide gel
electrophoresis and autoradiography will also be presented.

GUEST FACULTY:

John Ryan, University of Connecticut
Dr. Peter Del Vecchio, Albany Medical College
Dr. Wallace L. McKeehan, W. Alton Jones Cell Science Center
Dr. Martha Weinstein and Dr. Peter Benn, Diagenetics Laboratories
Dr. Michael Gabridge, Bionique Laboratories
Dr. James Haley, College of Mt. St. Vincent & Albert Einstein College
  of Medicine
Ellyn Fischberg-Bender, Roche Diagnostic Systems

Registration (lunch optional)     $850
Non-refundable reservation fee applicable to registration  $100

For additional information on the course schedule and on-campus
housing, contact the Course Director:

Dr. Frances M. Cardillo
Director, Biotechnology
Manhattan College
Mount St. Vincent Campus
Bronx, New York 10471
USA
Tel.: 212-549-8000 ext. 367

or e-mail:  Dr. Myrna Watanabe, scf7 at manvax.bitnet

REGISTRATION DEADLINE:  MAY 1, 1990

MANHATTAN COLLEGE AND THE COLLEGE OF MOUNT ST. VINCENT RESERVE THE
RIGHT TO CANCEL COURSES WITH INSUFFICIENT ENROLLMENT.



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