PCR machine

Rick K. Wilson rkwilson at JULIET.CALTECH.EDU
Thu Mar 8 23:51:02 EST 1990


In answer to J. Kruper of the University of Chicago:

Believe it or not, a microprocessor-controlled convection oven, manufactured by
BioTherm (Arlington, VA) and marketed by BIOS (Bay area), is one of the best
devices for PCR presently available.  Price is around $3000, no mineral oil is
necessary, oven can hold up to 200 microfuge tubes or four polypropylene 96-well
plates (Costar).  Reproducibility from run to run seems much better than with
our three Perkin-Elmer Cetus machines (at $8000 a pop!).  The only disadvantage
of the oven is that cycle times tend to be longer, although when you consider
the increase in sample capacity, longer cycles are not that bad of a trade-off.

If you're looking for a useful, reliable and relatively inexpensive system for
PCR, I highly recommend that you take a look at this device.

Richard K. Wilson, Ph.D.
Division of Biology, 147-75
Caltech
Pasadena, CA   91125
(818) 356-4938




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