Extinction Coefficients Revisted...

Keith Elliston elliston at av8tr.UUCP
Thu Oct 18 09:03:47 EST 1990


Hello again...

I recently posted a question on calculating protein extinction coefficients.
I received a couple good replies, but I actually got the best information 
over the phone from Irv Edelman at GCG.  He recommended a paper by Gill
and von Hippel (Anal. Biochem. 182:319-26. 1989) which goes into the both
the calculation on extinction coefficients at 280nm, as well as their 
significance WRT the real experimentally determined values.

I then used their calculation (quite simple in fact) to write a little
snippet of code that I put into the GCG program peptidesort.  Now I have
a little program that calculates extinction coefficients as well as all
the other nice things peptidesort did before.

If any one is interested in my little snippet of code, drop me a line and
i can send it.  According to Irv, Peptidesort will do these calculations
in release 7, so don't feel that you need to modify your version right away
;)

Thanks again for the info...

Keith
uunet!av8tr!elliston

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Keith O. Elliston       |    .signature really under construction....   
uunet!av8tr!elliston    |        
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