REBASE info

[Dana Macelis at Cold Spring Harbor Laboratory]

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                    DR. RICHARD J. ROBERTS' REBASE
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		         N E W     I N F O 

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SEVERAL NEW FILES ARE AVAILABLE:

 format #   format name
 --------   -----------
       20.  Commercial Restriction Enzyme Info
       21.  All Types I, II & III (parsed references)  
       22.  All Types I, II & III (sorted by microorg)  
       23.  Commercial Methylase Listing
       24.  Commercial Methylase Info  



These can be received on a monthly basis via email or retrieved from our 
anonymous ftp site.


descriptions follow:

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20.         ---  COMMERCIAL RESTRICTION ENZYME INFORMATION  ---
----------------------------------------------------------------------------
DESCRIPTION:   a list of companies from which restriction enzymes are 
               available, their address information, and the enzymes 
               that they sell.  (combines information in formats 17 & 18).


-------------------------------------------------------------------------------
21.                 ---   ALL ENZYMES (PARSED REFERENCES)   ---
-------------------------------------------------------------------------------
DESCRIPTION:   A list of all restriction enzymes - Types I, II, and III. 
               In this report, references are listed in a parsed format.

EXAMPLE:

<ENZYME NAME>
<PROTOTYPE>
<MICROORGANISM>
<SOURCE>
<RECOGNITION SEQUENCE>
<METHYLATION SITE>
<COMMERCIAL AVAILABILITY>


<1>CviJI
<2>
<3>Chlorella strain NC64A (IL-3A)
<4>J.L. Van Etten
<5>RG^CY
<6>3(5)
<7>
<8>1,603

<1>DdeI
<2>
<3>Desulfovibrio desulfuricans Norway strain
<4>H. Peck
<5>C^TNAG
<6>1(5)
<7>BEGIJLMNOPRUVX
<8>177,232,397

<1>XhoI
<2>
<3>Xanthomonas holcicola
<4>ATCC 13461
<5>C^TCGAG
<6>
<7>ABDEFGHIJKLMNOPQRSUVX
<8>187

<1>XmaI
<2>SmaI
<3>Xanthomonas malvacearum
<4>ATCC 9924
<5>C^CCGGG
<6>
<7>DEINRUVX
<8>152

References:

1.	<AUTHORS>Agarwal, K.	<U>	unpublished observations.
2.	<AUTHORS>Aiken, C., Milarski-Brown, K., Gumport, R.I.	<J>	        <YEAR>1986	<JOURNAL>Fed. Proc.	<VOLUME>45	<PAGES>1914
3.	<AUTHORS>Akulinin, G.E., Getko, G.A., Repin, V.E., Degtyarev, S.K.	<J>	<YEAR>1988	<JOURNAL>Izv Sib Otd Akad. Nauk SSSR Ser Biol Nauk	<VOLUME>14	<PAGES>105-108

4.	<AUTHORS>Arrand, J.R., Myers, P.A., Roberts, R.J.	<U>	unpublished observations.


-------------------------------------------------------------------------------
22.                 ---   ALL ENZYMES (SORTED BY ORGANISM)   ---
-------------------------------------------------------------------------------
DESCRIPTION:   This is a complete list of enzymes currently stored in the
               database.  Each enzyme is followed by its reference information.
               The enzymes are sorted by their microorganism names.
EXAMPLE:

<ENZYME NAME>
<PROTOTYPE>
<MICROORGANISM>
<SOURCE>
<RECOGNITION SEQUENCE>
<METHYLATION SITE>
<COMMERCIAL AVAILABILITY>
<REFERENCES>

<1>ApaI
<2>
<3>Acetobacter pasteurianus sub. pasteurianus
<4>NCIB 7215
<5>GGGCC^C
<6>4(5)
<7>BDEGIJKLMNOPRUVX
<8>Seurinck, J., Van de Voorde, A., Van Montagu, M. A new restriction endonuclease from Acetobacter pasteurianus.  Nucl. Acids Res. 11: 4409-4415 (1983)
Trautner, T.A. unpublished observations.

----------------------------------------------------------------------------
23.                 ---  COMMERCIAL METHYLASE LISTING  ---
----------------------------------------------------------------------------
DESCRIPTION:    Listing of which methylases are currently available
		from each company in the database.

----------------------------------------------------------------------------
24.                ---  COMMERCIAL METHYLASE INFORMATION  ---
----------------------------------------------------------------------------
DESCRIPTION:   a list of companies from which methylases are available,
               their address information, and the methylases that they sell.
               (combines information in formats 18 & 23).


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