Tet resistance

BI1RGW at IBM.SHEFFIELD.AC.UK BI1RGW at IBM.SHEFFIELD.AC.UK
Thu Dec 12 06:16:23 EST 1991


The tetracycline resistance gene on pBR322 and that on transposon Tn10 are
different, so (clearly) there are at least 2 different resistance genes.
It is possible to select against tet-resistance due to Tn10 using
Fusaric Acid and ZnCl2, using heat-denatured chlortetracycline to induce
the resistance. This procedure works because of a reduction in the
membrane's permeability to Tet, which makes the membrane *more* permeable
to Fusaric Acid.
BUT, this selection doesn't work against pBR322. So, presumably, the tet
resistance on pBR322 doesn't work in the same way.

Hope this is of some help,

Robin Walters                    Robert Hill Institute,
                                 Dept. of Mol. Biol. & Biotech,
BI1RGW at IBM.SHEF.AC.UK            Univ. of Sheffield,
                                 U.K.



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