restriction recognition sites in mammalian genome

THEMMEN%HROEUR51 at PUCC.PRINCETON.EDU THEMMEN%HROEUR51 at PUCC.PRINCETON.EDU
Mon Feb 25 08:07:00 EST 1991


Thomas Collet <collet at SCRIPPS.EDU> writes:


>I am trying to clone immunoglobulins from hybridoma cells and
>am wondering whether I am using the best possible restriction
>enzymes.  Does anyone have a compilation of the cutting
>frequencies of restriction enzymes in immunoglobulin genes (or
>maybe just mammalian genes in general ?) Or maybe a literature
>reference ?
>Thanks
>
>Thomas

Thomas,

The restriction site frequency can vary with the restriction enzyme's
recognition pattern. In the New England Biolabs Catalogue you (88-89, p. 141
will find some info on the cutting frequency of some enzymes (NotI:
(approx fragment size: 1000 kb) MluI, NruI, PvuI (300 kb) SfiI, RsRII
(200 kb) SacII, BssHII, EagI, SmaI, SalI, AatII, XhoI, FspI, SnaBI, ClaI,
AsuII (100 kb). As you can see all these enzymes have one or more CG's in
their recognition seq. The above frequencies hold true for the
mammalian genomes.

Regards,
Axel Themmen (Themmen at HROEUR51.bitnet)




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