restriction enzyme sites in mammalian cell

Thomas Collet collet at SCRIPPS.EDU
Fri Feb 22 18:38:26 EST 1991

I am trying to clone immunoglobulins from hybridoma cells and
am wondering whether I am using the best possible restriction
enzymes.  Does anyone have a compilation of the cutting
frequencies of restriction enzymes in immunoglobulin genes (or
maybe just mammalian genes in general ?) Or maybe a literature
reference ?


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