restriction enzyme sites in mammalian cell

[BIOCOL at UNIPAD.UNIPD.IT]

In BioNews Thomas Collet says:

>I am trying to clone immunoglobulins from hybridoma cells and
>am wondering whether I am using the best possible restriction
>enzymes.  Does anyone have a compilation of the cutting
>frequencies of restriction enzymes in immunoglobulin genes (or
>maybe just mammalian genes in general ?) Or maybe a literature
>reference ?
>Thanks

I think you can try by yourself using stochastic computer methods (as I did),
for example RND in BASIC. The frequence of cutting for each enzyme must be
1/4 exp n for each class with n bases of recogniction. I have no litterature
but if you want I can send you, with hard mail, some table for 6 and 4 base-cut
ters.

Best Re: and Re:gards.                           Argenton Francesco
                                                  Dip. di Biologia
                                                Universita' di Padova
                                                Via Trieste 75, 35100
                                                   Padova, ITALIA