PCR blunt end subcloning

Hirdeypal S. Bhathal hsb at chem.ucsd.edu
Thu Oct 17 17:40:56 EST 1991


I tried to subclone RACE PCR products into Hinc II site of pUC-19 after
blunt ending them with T4 polymerase. On gel, it seems that the ligation worked 
but I never got a white colony. I am planning to do PCR with primer having
Eco RI site. I'll appericiate if anyone can give some suggestion.
Thanks

Hirdeypal
hsb at chem.ucsd.edu
   



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