TYRODES ORIGINAL REF.

[Kip D. Hauch]

OK  stupid question time.

IUve seen the name TyrodeUs solution and TyrodeUs buffer
(and of course more frequently HEPES-TyrodeUs..) slapped
on many many different formulations of balanced salt solutions.
Everybody adds a touch of CaCl2 here, ATP there, MgCl2 here, etc.

Q:  Has *TyrodeUs solution* become a euphemism for an iso-osmotic NaCl
solution or is there really a formulation ascribed to it?

Can anyone point me to an ORIGINAL TyrodeUs solution/buffer formulation
reference if there is one?

Anyone have an ORIGINAL reference as to how HEPES was added to this, and
what it replaced (assume carbonate)?  

Is there a consensus as to what this name/term (TyrodeUs) means and how it
should be used?


Feel free to E-MAIL me your favorite recipe for TyrodeUs or HEPES-TyrodeUs
from your local labs recipe files along with refs.  I will summarize and
post if sufficient replies are received.