pUC119 : Info wanted.

[Keith Robison]

wetsel_r at wums.wustl.edu writes:

>In a previous article, proberts at castle.ed.ac.uk (P Roberts) wrote:
>>I'm looking for information on the plasmid pUC119.  None of the
>>databases to which I have access have heard of it.  Does anyone out
>>there have any information they could let me have?  I would like to know
>>things like marker (presumably ampicillin), polylinker and any other
>>bits and bobs that it has.  A complete sequence would be great if
>>someone has a copy on-line that they could mail me.
>>  Thanks very much for any help you can offer.
>> 
>>          Paul (proberts at uk.ac.ed.castle)
>----=
>Paul:

>Forgive the stupid question... are you sure it's pUC119 as in *119*????  
>I've heard of pUC19, as in *19*, but not 119.  If it is in fact pUC19 that 
>you need, I think I can provide it to you but having been in mol-bio for 5 
>years, I've never heard of pUC119.

I believe that you will find that pUC119 DOES exist -- it is pUC19 with
an M13 ori added to convert it to a phagemid.


Keith Robison
Harvard University
Department of Cellular & Developmental Biology
Department of Genetics / HHMI

robison at biosun.harvard.edu