t-RNAs and protein degradation
Donald Chen - Microbiology
chend at ucs.orst.edu
Tue Oct 27 01:21:47 EST 1992
I've just come across two papers which discuss eukaryotic
degradation via ubiquitin. These are by Ciechanover et al
PNAS 82:1341-1345 (1985) and Ferber and Ciechanover
Nature 326:808-811 (1987). Both papers show that charged t-RNA (arg)
is necessary for protein degradation via the ubiquitin pathway.
I have some questions for those who might be in this field of
1. Have any of the authors continued to look into the role of
t-RNA in ubiquitin mediated proteolysis?
2. Is there in vivo evidence of t-RNA charging of cellular
proteins which are destined for degradation?
3. Is there a prokaryotic version of t-RNA and proteolysis?
Yes, I know that there is no evidence of an ubiquitin-like
molecule in prokaryotes. But do t-RNAs have any role
in prokaryotic proteolysis?
4. I have learned that t-RNAs may have a role in transcription
by RNA pol III. Are there other roles for t-RNAs other than
translation in either eukaryotes or prokaryotes?
5. If t-RNAs are involved in marking/facilitating ubiquitin
mediated proteolysis where are these t-RNAs coming from-
the nucleus? Does ubiquitin work in the nucleus as well as
Any help would greatly be appreciated.
Oregon State Univ
Dept of Microbiology
More information about the Bioforum