antibody fluorescence

Mark S. kh at litsun17.epfl.ch
Mon Sep 28 16:06:08 EST 1992


Trp fluorescence is dependent on the micro environment. Going from an aqueous surrounding (as in solution) to a more hydrophobic one (as in bound to lipids or within a protein surrounding there are two measurble changes: the fluorescence quantum yield goes up, probably because the exited state looses a raditionless relaxation mechanism thru contact with water molecules. Moreover there is a blue shift in the emision (250 -> 220 nm, depending on hydrophobicity), I think because the exited state is polar and 






has a higher energy in a hydrophobic environment.

If you want to use quenchers: there are a lot of them, both aqueous and hydrophobic. 


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