Restriction enzyme help
nash at biologysx.lan.nrc.ca
Fri Apr 2 13:30:11 EST 1993
In article <1993Apr2.181617.15803 at ramsey.cs.laurentian.ca> abigail at ramsey.cs.laurentian.ca (Shawn Abigail) writes:
>From: abigail at ramsey.cs.laurentian.ca (Shawn Abigail)
>Subject: Restriction enzyme help
>Summary: Restriction enzyme help
>Keywords: restriction enzyme
>Date: Fri, 2 Apr 1993 18:16:17 GMT
>I downloaded a file with a list of restriction enzymes, their sequences
>and sources. I am a little confused over the nomenclature of the sequences.
>C, T, A & G are obvious! R stands for a Purine and Y stands for a
>Pyrimidine. Does N stand for "any nucleotide"? What about W and S?
>Any help is gratefully appreciated.
>abigail at ramsey.cs.laurentian.ca
R = A or G Y = T or C
W = A or T S = G or C
M = A or C K = G or T
H = A or T or C
B = C or T or G
V = A or C or G
D = A or G or T
N = A or C or G or T
I think I got it right!
John Nash | Email: Nash at biologysx.lan.nrc.ca.
Institute for Biological Sciences, | National Research Council of Canada,
Cell Physiology Group. | Ottawa, Ontario, Canada.
*** Disclaimer: All opinions are mine, not NRC's! ***
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