Restriction enzyme help

Martin Kennedy cytogen at chmeds.ac.nz
Mon Apr 5 17:48:49 EST 1993


In article <nash.88.0 at biologysx.lan.nrc.ca>, nash at biologysx.lan.nrc.ca (John Nash) writes:
> In article <1993Apr2.181617.15803 at ramsey.cs.laurentian.ca> abigail at ramsey.cs.laurentian.ca (Shawn Abigail) writes:

>>I downloaded a file with a list of restriction enzymes, their sequences
>>and sources.  I am a little confused over the nomenclature of the sequences.
>>C, T, A & G are obvious!  R stands for a Purine and Y stands for a
>>Pyrimidine.  Does N stand for "any nucleotide"?  What about W and S?
>>Any help is gratefully appreciated.
> 
>>Shawn Abigail
>>abigail at ramsey.cs.laurentian.ca
> 
> R = A or G	Y = T or C
> W = A or T	S = G or C
> M = A or C	K = G or T
> H = A or T or C
> B = C or T or G
> V = A or C or G
> D = A or G or T
> N = A or C or G or T
> 
> I think I got it right!
> 
> cheers, John
> 

Some very helpful mnemonics for these characters have just been posted on
bionet.methds-reagnts if you are interested.
- 

Cheers,

Martin

Martin A Kennedy (E-mail = cytogen at chmeds.ac.nz)    
Cytogenetic and Molecular Oncology Unit               
Christchurch School of Medicine                       
Christchurch, New Zealand                     



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