Restriction enzyme help
cytogen at chmeds.ac.nz
Mon Apr 5 17:48:49 EST 1993
In article <nash.88.0 at biologysx.lan.nrc.ca>, nash at biologysx.lan.nrc.ca (John Nash) writes:
> In article <1993Apr2.181617.15803 at ramsey.cs.laurentian.ca> abigail at ramsey.cs.laurentian.ca (Shawn Abigail) writes:
>>I downloaded a file with a list of restriction enzymes, their sequences
>>and sources. I am a little confused over the nomenclature of the sequences.
>>C, T, A & G are obvious! R stands for a Purine and Y stands for a
>>Pyrimidine. Does N stand for "any nucleotide"? What about W and S?
>>Any help is gratefully appreciated.
>>abigail at ramsey.cs.laurentian.ca
> R = A or G Y = T or C
> W = A or T S = G or C
> M = A or C K = G or T
> H = A or T or C
> B = C or T or G
> V = A or C or G
> D = A or G or T
> N = A or C or G or T
> I think I got it right!
> cheers, John
Some very helpful mnemonics for these characters have just been posted on
bionet.methds-reagnts if you are interested.
Martin A Kennedy (E-mail = cytogen at chmeds.ac.nz)
Cytogenetic and Molecular Oncology Unit
Christchurch School of Medicine
Christchurch, New Zealand
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