antisense oligos (fwd)

MJ Duggan sgex400 at uk.ac.lon.sghms
Tue Aug 24 06:18:28 EST 1993


Dear netters,

I posted this question in the methods net but got no replies so I thought
that maybe I would try putting it in the general circulation to get some
help.

If I get any useful replies I will post a summary.

Thanks

Michael

> 
> Hello,
> 
> I want to use antisense oligonucleotides to inhibit the synthesis of my
> favourite protein in cell culture. A previous reference used all
> phosphorothionate linkages, but this makes the whole procedure very
> expensive. I have heard that using thiol substitutions at all points is
> not really necessary, but that using substitutions at the terminal
> couplings only will prevent exonuclease digestion, which is the major
> degredative pathway. Is this true? Has anybody done this and shown that the
> different oligos are equipotent or nearly so?
> 
> Any advice gratefully received,
> 
> michael duggan
> 
> 8-}
> 
> 
> 
> 



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