antisense oligos (fwd)

MJ Duggan sgex400 at
Tue Aug 24 06:18:28 EST 1993

Dear netters,

I posted this question in the methods net but got no replies so I thought
that maybe I would try putting it in the general circulation to get some

If I get any useful replies I will post a summary.



> Hello,
> I want to use antisense oligonucleotides to inhibit the synthesis of my
> favourite protein in cell culture. A previous reference used all
> phosphorothionate linkages, but this makes the whole procedure very
> expensive. I have heard that using thiol substitutions at all points is
> not really necessary, but that using substitutions at the terminal
> couplings only will prevent exonuclease digestion, which is the major
> degredative pathway. Is this true? Has anybody done this and shown that the
> different oligos are equipotent or nearly so?
> Any advice gratefully received,
> michael duggan
> 8-}

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