Ames Assays and E. coli

William A. Rosche WROSCHE at IBT.TAMU.EDU
Mon Feb 8 14:20:56 EST 1993


To all concered:

    Are there E. coli strains with the same mutations as the Ames
Salmonella tester strains?

    In a recent artical (Sofuni,T. Envirnomental and Molecular
Mutagenesis, vol. 21, p. 2-7), Fig. 1 shows data from another study
of Matsushima ("Mutation and the Environment" Wiley-Liss, New York.
p. 251-255, 1990) which compares the response of 475 compounds in an
E. coli and Salmonella reversion mutagenesity assay.  The results
were as follows
        Positive in Salmonella  325
        Positive in Both        115
        Positive in E. coli      35

  I would like to know if these differences are due to different loci
being tested, or different response of E. coli vs. Salmonella.  If
someone knows if experiments have been done at the same locus in
these two different bacteria, I would like to know.  I will assume
that these experiments must be done on plasmids, because chromosomal
location should matter.

    Please respond directly to my e-mail, and I will post a summary
of responses.

    Thank you in advance,
            William A. Rosche

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William A. Rosche
Graduate Assistant Researcher

Center for Genome Research
Insitute of Biosciences and Technology
Texas A&M University at the Texas Medical Center
2121 Holcombe Blvd
Houston, TX 77030-3303
(713) 677-7672
(713) 677-7689 FAX

<wrosche at ibt.tamu.edu>
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