HELP-RNA FROM PINE NEEDLES?!

parkes at waikato.ac.nz parkes at waikato.ac.nz
Sun Feb 14 18:46:18 EST 1993


I'm a masters student doing molecular biology at Waikato.NZ and have been
trying to make a cDNA library from Pinus radiata needles for what seems years
but has been several months, one of several I'm ment to make for the project of
finding a leaf specific promotor.

Does ANYBODY know a method or alteration that may allow me to get a resonable
conc. of RNA for the library? I've only got low yeilds of undegraded of more
often degraded RNA.

Any help/advice/protocol would be most welcome.

Yours sincerely 

Bryan Parkes.



More information about the Bioforum mailing list