Cell Imaging System
Dr Jim Cummins
cummins at CSUVAX1.MURDOCH.EDU.AU
Sun Jun 6 19:27:02 EST 1993
On 2 Jun 1993, Mohammed Kazam Najafi Koopai wrote:
> Hi everybody,
> I am working on a project that involves biological
> cell image analysis. Our application requires a real time image
> processing system that, given the microscopic images of the
> experimented cell, outputs the centroid position and orientation
> of the cell. This information is then used to measure DiElectroPhoresis
> characteristics of the cell.
> The system contains a microscope which is focused on the cells
> floating in a transparent chamber. A CCD camera grabs the microscopic
> image at the rate of 30 frames/second. An image processing block is
> needed to get the image and calculates the centroid of the floating
> cell at the same rate. The orientation of the cell is also required
> to be found. The orientation of the cell is needed to measure the
> rotational speed of the cell. In our application, there are many
> cases in which the cell is spinning in its place and the spinning rate
> is desired.
> Currently, an image processing hardware made by Matrox Company, Quebec,
> Canada (Imager-AT 1987) together with a software is being used.
> However, the following difficulties in our system have been encountered
> so far:
> - Although the spherical cells are chosen for this experiments,
> the cell images obtained are not always spherical. The non-
> uniform density of the inner part of the cell is the main
> reason for this appearance. This appearance causes faulty
> detections of the centroid position. The image analysis
> system has to reconstruct the real shape of the cell before
> centroid detection.
> - The process is not fast enough to detect quick cell movements.
> - The frames with multiple cells yield erroneous centroid
> position. The system cannot distinguish between the cells.
> We might have to use a faster hardware.
> An alternative solution to this problem can be using Analog
> Position Sensor ICs. But I do not know of the ICs available or
> where to start looking for these ICs.
> I would appreciate if anybody can help me or give some pointers in this
> regard. If you know of anybody working on a similar project please let
> me know. Please send me E_mail at the following address:
> najafi at enel.ucalgary.ca
> Thank you in advance.
> Kazem Najafi
Check out the various systems that are now available for looking at sperm
motion analysis. Hamilton Thorn Research has a new system that uses 60f/s
video and has a number of advanced options for specifying motion
parameters. I'm not sure whether it would be able to handle your
particular problems, though. Email me directly if you want further info.
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