Summer competition

Bruce Roe broe at aardvark.ucs.uoknor.edu
Mon Jun 7 09:11:00 EST 1993


In article <1993Jun7.111420.91104 at embl-heidelberg.de>, rice at embl-heidelberg.de (Peter Rice) writes...
>No prizes, but a challenge to the net:
> 
>The following posting just appeared on rec.humor.funny. Of course it is
>nonsense, but the task is to suggest a sequence that would make the
>joke work after all.
> 

Hi all,
	Just for fun, I did a tblastn search of the nonredundant database
using the peptide sequence "THISSPACEINTENTIONALLYLEFTBLANK"

The results are interesting?   ;-)

Wonder what Grail and GeneID give.

Cheers.......bruce
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
 \  Bruce A. Roe                     Dept. Chemistry and Biochemistry /
 /  BROE at aardvark.ucs.uoknor.edu     University of Oklahoma           \
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

                                                                     Smallest
                                                                     Poisson
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N

gb|X14593|PHCHSD     P.hybrida chsD gene for chalcone ... -3    37  0.024     2
gb|X62395|NTLTP1     N.tabacum ltp1 gene for lipid tra... +1    36  0.030     2
gb|X62460|ATH12FLK   A.thaliana gene for histone H1-2f... -1    37  0.031     2
gb|X54850|SKORFS     S.kluyveri linear plasmid pSKL DN... -2    38  0.063     2
gb|X17693|ISTN7TNS   Transposon Tn7 transposition gene... -2    37  0.068     2
gb|D11079|VACRHF     Vaccinia virus genomic DNA, 42kbp... +1    40  0.30      2
gb|L18073|HUMUT2437  Human STS UT2437.                    +1    51  0.34      1
gb|Y00795|DMMP20     Drosophila melanogaster mp20 gene... +1    51  0.44      1
gb|Z19157|CEZC84     Caenorhabditis elegans cosmid ZC8... +2    45  0.47      2
gb|M77697|CELB0303   C.elegans cosmid B0303. >emb|M776... +3    40  0.48      2
gb|M86409|HSV3PRGEN  Herpesvirus saimiri the most thre... +2    38  0.50      2
gb|M87049|ECOUW85U   E. coli. genomic sequence of the ... +1    38  0.54      2
gb|L03534|ENHMHCAX   Entamoeba histolytica myosin heav... +2    50  0.56      1
gb|M16748|HUM14INV3  Human T-cell receptor centromeric... -3    39  0.64      1
emb|S70552|S70552    tRNATyrGUA [Dictyostelium discoid... -2    39  0.64      1
gb|X55434|AMTAP2     Antirrhinum majus tap2 gene for T... -3    49  0.66      1
gb|M75730|RSHPOLLA   Human respiratory syncytial virus... -2    49  0.68      1
gb|M74568|RSHSEQ     Human respiratory syncytial virus... -1    49  0.68      1
gb|M10479|HUMRSA     Human repetitive sequence alpha. ... -3    38  0.71      1
gb|M64239|MUSTCRA    Mouse T-cell receptor alpha/delta... -3    36  0.78      2
gb|M55672|NEUHSP30   Neurospora crassa heat shock prot... +2    48  0.78      1
gb|X64521|HHFEROXI   H.halobium gene for pyruvate:ferr... -1    48  0.78      1
gb|M35027|VACCG      Vaccinia virus, complete genome. ... +2    40  0.80      2
gb|M29655|HUMSAU3A   Human Sau3 repeat extrachromosoma... -2    46  0.82      1
gb|X64346|HSGEND     Herpesvirus saimiri complete geno... +3    38  0.83      2
gb|X01704|GMNOD23    Soybean nodulin-23 gene >emb|X017... +3    47  0.87      1
gb|L08107|HUMGFCIIA  Human low-affinity Fc-gamma recep... -1    47  0.88      1
gb|L08109|HUMGFCIIC  Human low-affinity Fc-gamma recep... -3    47  0.88      1
gb|M23582|M23582     Figure 3. Partial nucleotide sequ... -3    38  0.89      1
gb|M57962|APLELH18   Aplysia californica egg-laying ho... +1    46  0.90      1
gb|Z12009|HSASP1119  H.Sapiens alpha satellite DNA >em... -3    46  0.93      1
gb|M93288|HUMSATAAC  Human alpha-satellite repetitive ... +2    46  0.93      1
gb|X71001|HSAREP1    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71072|HSAREP10   H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71073|HSAREP11   H.sapiens alphoid repetitive DNA ... -1    45  0.93      1
gb|X71074|HSAREP12   H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71075|HSAREP13   H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71076|HSAREP14   H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71002|HSAREP2    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71003|HSAREP3    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71004|HSAREP4    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71005|HSAREP5    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71007|HSAREP7    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|X71008|HSAREP8    H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|M20433|HUMRSCNTL  Human alphoid satellite DNA repea... -2    45  0.93      1
gb|M16087|HUMSATA7B  Human dimeric centromeric alpha-s... -2    45  0.93      1
gb|X71077|HSAREP15   H.sapiens alphoid repetitive DNA ... -2    45  0.93      1
gb|M18119|HUMRSCNTE  Human alphoid repeat (alpha-RI-DN... -3    45  0.93      1
gb|M93286|HUMSATAAA  Human alpha-satellite repetitive ... +3    46  0.93      1
gb|M77957|M77957     EST01541 Homo sapiens cDNA clone ... +2    45  0.94      1
gb|M36407|CUC11SGB   Pumpkin 11-S globulin beta-subuni... -2    46  0.94      1
emb|X64108|OSWAX     O.sativa waxy gene >gb|X64108|OSW... +2    46  0.95      1
gb|L15204|HUMSWX102  Human chromosome X STS sWXD102; s... -3    43  0.96      1
gb|M65284|HUMCFTRF1  Human cystic fibrosis transmembra... -3    42  0.97      1
gb|M33958|CRUPRPA    Chinese hamster prion protein (Pr... +3    45  0.97      1
gb|M98788|HUMD7S476X Human dinucleotide polymorphism a... +1    43  0.97      1
gb|L01710|CHPRSAAH   Chimpanzee alphoid repetitive seq... -2    44  0.97      1
gb|L01716|CHPRSAAN   Chimpanzee alphoid repetitive seq... -2    44  0.97      1
gb|M58800|KURRR16S   Kurthia zopfii 16S ribosomal RNA.... -2    45  0.98      1
gb|M28428|YSCTUB3    S.cerevisiae alpha-tubulin (TUB3)... +1    45  0.98      1
gb|M92099|MUSMYH     Mouse myosin heavy chain (MYH) ge... -3    45  0.98      1
gb|X14440|HSXCHA     H.sapiens X chromosomal gene for ... +3    45  0.98      1
gb|X04336|MIPAIVS2   Podospora anserina race A mitocho... -3    45  0.98      1
gb|M57517|SYCCPN60   Synechocystis sp. chaperonin 60 (... +2    45  0.98      1
gb|X62169|ECINCI2    E.coli IncI2 plasmid R721 pilU, p... +3    45  0.98      1
gb|X55026|MTPACG     P.anserina complete mitochondrial... -3    45  0.98      1



>gb|X14593|PHCHSD P.hybrida chsD gene for chalcone synthase >emb|X14593|PHCHSD
            P.hybrida chsD gene for chalcone synthase
            Length = 2948

  Minus Strand HSPs:

 Score = 37 (16.9 bits), Expect = 52., P = 1.0
 Identities = 6/9 (66%), Positives = 7/9 (77%), Frame = -3

Query:     6 PACEINTEN 14
             PACE+ T N
Sbjct:  1164 PACEMKTRN 1138

 Score = 36 (16.4 bits), Expect = 71., Poisson P(2) = 0.024
 Identities = 6/14 (42%), Positives = 8/14 (57%), Frame = -3

Query:     8 CEINTENTINALLY 21
             C IN +N  N  +Y
Sbjct:  1320 CNINCKNRFNNTIY 1279


>gb|X62395|NTLTP1 N.tabacum ltp1 gene for lipid transferase >emb|X62395|NTLTP1
            N.tabacum ltp1 gene for lipid transferase
            Length = 2075

  Plus Strand HSPs:

 Score = 36 (16.4 bits), Expect = 71., P = 1.0
 Identities = 5/21 (23%), Positives = 13/21 (61%), Frame = +1

Query:     6 PACEINTENTINALLYLEFTB 26
             P CE+++   +N+ + L+  +
Sbjct:  1156 PHCEMDSNKPLNSAMGLKLRD 1218

 Score = 35 (16.0 bits), Expect = 97., Poisson P(2) = 0.030
 Identities = 7/20 (35%), Positives = 14/20 (70%), Frame = +3

Query:     3 ISSPACEINTENTINALLYL 22
             ISSP+  +++ N++   L+L
Sbjct:   243 ISSPS*ILHSTNSLQLFLFL 302


>gb|X62460|ATH12FLK A.thaliana gene for histone H1-2flk (partial)
            >emb|X62460|ATH12FLK A.thaliana gene for histone H1-2flk (partial)
            Length = 2106

  Minus Strand HSPs:

 Score = 37 (16.9 bits), Expect = 52., P = 1.0
 Identities = 7/18 (38%), Positives = 11/18 (61%), Frame = -1

Query:     4 SSPACEINTENTINALLY 21
             S  +CEI+ E ++  L Y
Sbjct:   645 SQTSCEISAETSL*LLFY 592

 Score = 35 (16.0 bits), Expect = 97., Poisson P(2) = 0.031
 Identities = 7/11 (63%), Positives = 8/11 (72%), Frame = -2

Query:     1 THISSPACEIN 11
             TH SSPA  +N
Sbjct:   593 THYSSPAYIVN 561


>gb|X54850|SKORFS S.kluyveri linear plasmid pSKL DNA for open reading frames
            1-10. >emb|X54850|SKORFS S.kluyveri linear plasmid pSKL DNA for
            open reading frames 1-10.
            Length = 14,281

  Minus Strand HSPs:

 Score = 38 (17.3 bits), Expect = 38., P = 1.0
 Identities = 6/18 (33%), Positives = 14/18 (77%), Frame = -2

Query:    10 INTENTINALLYLEFTBL 27
             ++ EN I +++++ FT+L
Sbjct:  8682 LSLENEILSVIFILFTDL 8629

 Score = 37 (16.9 bits), Expect = 52., Poisson P(2) = 0.063
 Identities = 5/19 (26%), Positives = 12/19 (63%), Frame = -2

Query:     3 ISSPACEINTENTINALLY 21
             +++P C I   +T+N  ++
Sbjct:  6222 LTNPYCSILISDTVNKFIF 6166

 Score = 36 (16.4 bits), Expect = 71., Poisson P(2) = 0.11
 Identities = 6/17 (35%), Positives = 11/17 (64%), Frame = -2

Query:     8 CEINTENTINALLYLEF 24
             C +++E  +  L+YL F
Sbjct:  9084 CFMSSETELTLLIYLTF 9034


>gb|X17693|ISTN7TNS Transposon Tn7 transposition genes tnsA, B, C, D and E
            >emb|X17693|ISTN7TNS Transposon Tn7 transposition genes tnsA, B, C,
            D and E
            Length = 8351

  Minus Strand HSPs:

 Score = 37 (16.9 bits), Expect = 52., P = 1.0
 Identities = 7/14 (50%), Positives = 9/14 (64%), Frame = -2

Query:     8 CEINTENTINALLY 21
             CE NTE T   ++Y
Sbjct:  1582 CEENTEQTSPQIVY 1541

 Score = 36 (16.4 bits), Expect = 71., Poisson P(2) = 0.068
 Identities = 7/10 (70%), Po


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