Marsupial DNA/Library Needed
danj at welchgate.welch.jhu.edu
Wed Jun 16 08:20:55 EST 1993
In article <1vi11g$fhb at rigel.fccc.edu> brunk at cygnus.fccc.edu (Brian Brunk) writes:
>I am studying the evolutionary conservation of a mammalian enhancer element
>and would very much like to sequence a marsupial element. Anyone having or
>knowing of a marsupial genomic library or even DNA, ....
Point your gopher client at merlot.welch.jhu.edu and select the following:
--> 15. Searching For Biologists/
--> 2. Search for All Researchers funded by NIH <?>
Now search for your favorite marsupial -
opossum and dna
will yield several entries, one of which is as follows:
MIGEON, BARBARA R
JOHNS HOPKINS HOSPITAL
600N WOLFE ST
BALTIMORE, MD 21205-2196
PERFORMING ORGANIZATION: JOHNS HOPKINS UNIVERSITY
TITLE Genetic studies of human somatic cells
We plan to continue our investigations of X inactivation and
genomic imprinting in human females by exploring the molecular mechanisms.
As X inactivation is the means of X dosage compensation in all mammals and
features that differ among mammals are useful in dissecting components of
the mechanisms involved, we will continue studies of X inactivation in
marsupial and mouse as well as human cells.
1. Our previous studies of X inactivation in germ cells suggest that
gametic differences in DNA. methylation could provide the *molecular basis
for at least some phenomena attributed to parental differences in genomic
imprinting. We will extend these studies by examining the methylation
status of pre-meiotic and meiotically arrested female germ cells, and
primordial germ cells of both sexes. We will analyze methylation using
antibodies to 5-methylcytosine in histological preparations of gonads, and
develop a method for PCR amplification from small numbers of germ cells,
so as to examine specific HpaII sites. Using in situ hybridization we will
determine if methyltransferase is expressed in unmethylated cells.
2. Our studies of marsupial cells show inactivity of the paternal
G6PD allele despite absence of DNA methylation and raise the possibility
that an inhibitor complex may be silencing the G6PD
locus. We will look for such proteins using in vivo footprinting of the
G6PD-CpG island by, ligationmediated PCR.
3. We will explore in more detail the role of DNA replication in
reactivation and reversal of X
inactivation by examining replication of inactive and reactivated genes on
X chromosomes in mousehuman hybrid cells.
4. In search of sequences involved in early steps of X inactivation,
we will identify the X chromosome segment required for inactivation using
mouse-human hybrid cells containing X chromosomes with deletions in the
region considered to be the putative X inactivation center in mouse and
man. To identify the "essential" segment we will define the minimal area
of overlapping sequences present on deleted human X chromosomes that are
inactive, and the limits for the region of homology with the putative
inactivation center on the mouse X, and will look for conserved cDNAs,
expressed in early mouse embryos and mapping to this segment.
fluorescence activated cell sorter
polymerase chain reaction
early embryonic stage
sex linked trait
genetic disorder diagnosis
genetic registry /referral center
gene induction /repression
human population genetics
glucose 6 phosphate dehydrogenase deficiency
Lesch Nyhan syndrome
in situ hybridization
density gradient ultracentrifugation
tissue /cell culture
human genetic material tag
If you've never heard of gopher, don't worry, it's free and on the net.
Write me a note if you'd like information on how to get started.
A gopher search of genbank picks up a few marsupial entries - look up the
citations and call the authors.
AUTHORS Peacock,W.J., Dennis,E.S., Elizur,A. and Calaby,J.H.
TITLE Repeated DNA sequences and kangaroo phylogeny
JOURNAL Aust. J. Biol. Sci. 34, 325-340 (1981)
AUTHORS Mitchell,M.J., Woods,D.R., Wilcox,S.A., Graves,J.A. and Bishop,C.E.
TITLE Marsupial Y chromosome encodes a homologue of the mouse Y-linked
candidate spermatogenesis gene Ube1y.
JOURNAL Nature 359, 528-531 (1992)
AUTHORS Wainwright,B. and Hope,R.
TITLE Cloning and chromosomal location of the alpha- and beta-globin
genes from a marsupial
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 8105-8108 (1985)
AUTHORS Schneider,S., Vincek,V., Tichy,H., Figueroa,F. and Klein,J.
TITLE MHC class II genes of a marsupial, the red-necked wallaby (Macrepus
rufogriseus): Identification of new gene families
JOURNAL Mol. Biol. Evol. 8, 753-766 (1991)
AUTHORS Catanese,J.J. and Kress,L.F.
TITLE Opossum serum alpha-1-proteinase inhibitor: purification, linear
sequence and resistance to inactivation by rattlesnake venom
JOURNAL Biochemistry 33, 509-515 (1993)
AUTHORS Kaslow,D.C., Migeon,B.R., Persico,M.G., Zollo,M., VandeBerg,J.L.
TITLE Molecular studies of marsupial X chromosomes reveal limited
sequence homology of mammalian X-linked genes
JOURNAL Genomics 1, 19-28 (1987)
AUTHORS Koop,B. and Goodman,M.
TITLE Evolutionary and developmental aspects of two hemoglobin beta-chain
genes (epsilon-m and beta-m) of opossum
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 85, 3893-3897 (1988)
AUTHORS Juppner,H., Abou-Samra,A.-B.B., Freeman,M.W., Kong,X.F.,
Schipani,E., Richards,J., Kolakowski,L.F.Jr., Hock,J., Potts,J.T.,
Kronenberg,H.M. and Segre,G.
TITLE A G-protein linked receptor for parathyroid hormone related peptide
JOURNAL Science 254, 1024-1026 (1991)
Best of luck,
danj at welchgate.welch.jhu.edu
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