lambda gt11 antigenic proteins

delihas at ASTERIX.BIO.SUNYSB.EDU delihas at ASTERIX.BIO.SUNYSB.EDU
Wed Jun 16 09:30:58 EST 1993


	Does anyone have any information or experience identifying antigenic
proteins produced in lambda gt11 libraries?  These would be made from the lom
ORF, which is in the opposite direction to the lacZ ORF.  I was told that to screen for this, you should probe induced with IPTG plaques vs. non-induced.  The
difference in signal should determine if the protein you are probing for is 
antigenic or not (if there is no difference between induced or non-induced) 
Has anyone done this? Replies can be sent to delihas at asterix.bio.sunysb.edu.
		Thanks for all replies in advance.




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