Paraformaldehyde shelf-life?

M anon1167 at nyx.cs.du.edu
Fri Oct 22 15:56:33 EST 1993


I was recently told that, when using paraformaldehyde for 
perfusion, the P must be made immediately prior to perfusion.
I have been doing perfusions for years with para made up
to several days prior to the perfusion.  The people who 
have told me this don't seem to have a good reason _why_ you
need to make the stuff up right away.  Does para have a 
short shelf life?  Does "old" para react differently with tissue? 
(In this case, neural tissue?)  A

M



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