phage-display

rumbley catherine a crumbley at uxa.cso.uiuc.edu
Tue Oct 26 15:24:45 EST 1993


 I am looking for information about establishing a phage-display sysytem
 in our lab. This technique is new to us so we don't know where to start.
 We want to use phage-display to express single-cahin antibodies (about 26kD)
 and select for mutant antibodies which have higher binding affinties for
 their ligand and then sequence and propogate those clones.  Any information
 regarding phage-display would be greatly appreciated, including suggestions
 about which phage or system to use. 
 Thanks

 Cathy Rumbley
 crumbley at uxa.cso.uiuc.edu



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