PERCOLL GRADIENTS ANYONE??

Harald Osmundsen haraldo at karius.uio.no
Wed Sep 1 03:36:29 EST 1993


In article <259gfi$lup at bigguy.eng.ufl.edu>, armando at synapse.ee.ufl.edu (Armando Barreto) says:
>
>
>Hello, 
>
>I am working in the isolation of Kupffer Cells from rodent livers using Percoll  
>gradients. I am trying tu use the procedure developed by Bard Smedsrod and  
>Hakan Pertoft (1985) and having difficulty with the actual gracient formation.
>
>Has anyone successfully used this technique in isolating liver cells?
>
>Does anyone know of an e-mail address or phone number for the authors?
>
>Any experiences or suggestions will be greatly appreciated:
>
>armando at synapse.ee.ufl.edu
>
>Thank you.
>


Look in: Singh et al  Acta Physiol. Scand  (1983) 117, 497-505.

Here we used  self-generated Percoll-gradients to fractionate liver-cells. I worked for us - there is now reason why it should not work for you!


                    Harald Osmundsen            



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