alcohol dehydrogenase

[Marty Sachs]

In article <27l1k6$4v8 at news.umbc.edu> noronha at coe1.engr.umbc.edu (Who
wants to know?) writes:
>Hi,
> Are there any databases on inhibitors of enzymes?. I'm particularly
interested
>on inhibitors of alcohol dehydrogenase. 

We've used 4-methyl pyrazole (available from Sigma) with great success.

>Also, would anyone be aware of any work
>where the adh gene was deleted? I am working on a yeast biotransformation
>process where alcohol dehydrogenase is responsible for a lot of
byproduct, and
>hence would like to reduce its activity/delete the gene.

I assume you thinking specifically in yeast???  A selective agent (allyl
alcohol) used in maize to isolate ADH null mutations was first used in
yeast (Megnet, 1967, Arch. Biochem. Biophys. 121: 194), so I assume that
there are ADH null mutant strains in yeast.


          Marty Sachs
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