Common Forward Primers

Sean David Moore smoore at
Wed Aug 10 09:24:53 EST 1994

qRuari MacDonald (rem at wrote:

: I have been using RT-PCR to amplify two isozymes, M2- and L-type pyruvate
: kinase. Due to the reasonable high homology between these two isozymes i
: have been able to design a single common forward primer and 2 different
: reverse primers, producing two different length products. I'm slightly
: confused about the advantages of this method. Could someone please
: recommend some appropriate papers. Thank you in advance

: A confused Honours student

: :)

: Ruari

Sean Moore

VAMC Philadelphia,
Medical College of Pennsylvania,
and the University of Pennsylvania...

smoore at

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