Common Forward Primers

[Sean David Moore]

qRuari MacDonald (rem at uniwa.uwa.edu.au) wrote:

: I have been using RT-PCR to amplify two isozymes, M2- and L-type pyruvate
: kinase. Due to the reasonable high homology between these two isozymes i
: have been able to design a single common forward primer and 2 different
: reverse primers, producing two different length products. I'm slightly
: confused about the advantages of this method. Could someone please
: recommend some appropriate papers. Thank you in advance

: A confused Honours student

: :)

: Ruari

--
Sean Moore

VAMC Philadelphia,
Medical College of Pennsylvania,
and the University of Pennsylvania...
WORD BRO...I'M OUTTA HERE.
.

smoore at sas.upenn.edu