culturing splenocytes

Jeffrey Guy jsguy at
Fri Dec 9 23:22:42 EST 1994

I am trying to determine if a serum factor controls splenic
growth.   I've set up murine splenocytes in culture and then
add serum from experimental mice.   The question I have is
there any problem in using RPMI media with fetal bovine serum?
  Should I use a serum free media?   Please advise

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