HELP! Information Needed on TdT
asmsi002 at CMU.CHIANGMAI.AC.TH
Mon Feb 28 11:25:51 EST 1994
On 23 Feb 1994, John Ladasky wrote:
> I'm working with various protocols that involve the 3' end labeling
> of DNA with the enzyme TdT (terminal dexynucleotidyl transferase). I have
> not had a lot of success thus far, and I'd really like to get some informa-
> tion about the enzymology of TdT. All the articles I've turned up on Med-
> line have simply applied the enzyme, and not given information about the
> performance of the enzyme with various substrates. We're using biotin-11-
> dUTP, and we'd like to know what kind of behavior we should expect for TdT
> with it.
Please try to follow BMB(Boehringer Mannheim)'s Dig
Oligonucleotide Tailing Kit(cat. no. 1417231). In our hands, it works
impressively. If you can not find this protocol, please email to me.
No any conection with BMB.
Department of Clinical Microbiology
Faculty of Associated Medical Sciences
Chiang mai University
Email:asmsi002 at cmu.chiangmai.ac.th
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