Gram Stain

Norman Suguitan suguitan at
Fri Jul 1 20:37:24 EST 1994

When I flood a smear with crystal violet, is it better to just
concentrate on the smear, and don't bother dropping crystal
violet on the whole slide?

Secondly, how do I know when to stop decolorizing the stain.  Most
of the time, I just count 30 seconds and stop decolorizing.  Sometimes,
I could decolorize too much or not enough.  Do I decolorize the stain
directly, or just drop the alcohol on the side of the slide?

Another question.  What's the best technique in determining by looking
at a hanging drop slide, if the bacteria has motility, or movement seen
is just Brownian motion.  Do you actually see the bacteria moving?


suguitan at

More information about the Bioforum mailing list