Protein Recovery in Chromatography columns

The Zombie krishnam at umbc.edu
Wed Jun 15 09:54:54 EST 1994



What  are the typical recovery percentages of proteins from chromatographic columns?
i.e. If I were to carry out a Lowry assay on the protein peak collected from
the column, what percentage would it be of the protein I originally injected
into the column. A rough figure would suffice.

I am having this problem of low recovery percentages. I would greatly 
aprreciate any help in this regard.

please reply to :

krishnam at umbc7.umbc.edu

Thank you.

Rajesh krishnamurthy



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