Need info on micromanipulators

Frank Richardson FRICHARD at biomed.med.yale.edu
Sat Mar 5 11:58:44 EST 1994


In <2l5b4s$ob5 at senator-bedfellow.MIT.EDU> hkau at ATHENA.MIT.EDU writes:

> I'm considering adding micromanipulators and microinjectors to the 
> upright microscope I have. I found two vendors: Narishige and 
> Nikon.
> 
> Does someone have experience with these devices / companies? 

I have used the Narishige 3-D hydrolic fine micromanipulators for years. I do
electrophysiology so ZERO drift is necessary; the Narishige model does just
fine. I would recommend - depending on your specific needs - combining a course
manipulator for gross electrode positioning with the fine, hydrolic one for
delicate work. Narishige also makes a manual pressure, microinjector, but I
found this less than satisfying. If you want good control of injection look
into piscospritzers, i.e. something with a N2 gas line hooked up to it that can
be controled with a switch. Alternatively , if you are planning on injecting a
charged molecule (into cells), I recommend just filling an electrode with your
compound and then passing negative/positive current to eject it into the
cytoplasm (after cell impalement of course).

Virtually,
Frank


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frichard at biomed.med.yale.edu (vms/vax system)              
"Life is not easy for any of us. But what of that?"
 - Marie Curie
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