Pre-mRNA splicing-- kinetic control?

[gc]

In article <2lj17b$b4d at lace.Colorado.EDU>, eesnyder at beagle (Eric E. Snyder)
wrote:

> I have been trying to find a couple of references which support 
> or refute the following hypothesis:
> 
>     Pre-mRNA is or can be spliced differently depending on how quickly
>     it is synthesized.
> 
> I have developed an computer program (NAR 21(3): 607-613) which predicts 
> the intron-exon structure of human genes (and thus the splicing pattern)
> using a dynamic programming.  The algorithm implicitly assumes splicing
> starts with the whole transcript.  However, clearly the 3'-end of the 
> transcript is hanging out in space for a while before the transcript is
> finished.  The question is, does this make a difference?  Has anyone 
> seen any papers that address this possibility directly?  Lots of people
> claim that it might be true but I have never seen an experiment testing
> it...



If it helps any, I've seen scanning electron microsope pictures of what
are supposed to be snRNP complexes on RNA as it is still being transcribed.





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--gc