SEARCH RESULT FOR BLASTp from NCBI
robison1 at husc10.harvard.edu
Tue Mar 29 22:54:40 EST 1994
zainul at KB.USM.MY (Zainul Zain) writes:
>Hello, My name is Nazar. I',m stuck with this problem of interpreting
>the search result that was send back to me from NCBI. Could anybody give
>me some clue of what the "High Score" means etc.
> High Poisson
>Sequences producing High-scoring Segment Pairs: Score Probability
>sp|P11166|GTR1_HUMAN GLUCOSE TRANSPOTER TYPE 1, ERYTHR... 2505 0.0
>sp|P13181|GAL2_YEAST GALACTOSE TRANSPORTER (GALACTOSE P... 157 2.6e-25
>Could anybody tell me the significant of the two "High Score". I know
>that the one with the smallest Poisson Probability is one to look at.
>Actually, I have a lot to ask but let sort the above puzzle first. Thanks.
I would just point you to my little hypertext guide to sequence
searching (http://twod.med.harvard.edu/seqanal/), but I haven't added
the detailed explanation you are looking for!
BLAST produces ungapped local alignments. If there is more than
one high-scoring region, BLAST will return each of them. Hence
the "High Score" column -- it is the score of the highest scoring
local alignment. The Poisson score (sometimes) is calculated in
a way which counts all of the regions found.
Hope that helps...and I will update the guide.
Department of Cellular and Developmental Biology
Department of Genetics / HHMI
krobison at nucleus.harvard.edu
More information about the Bioforum