Black ECL blots

James Stiehr jcim at nyc.pipeline.com
Tue Apr 4 12:05:17 EST 1995


Has anyone else ever had problems using polyclonal antisera on a western
blot detected with ECL resulting in an overall black blot.  It is almost as
if the blots were not blocked at all (they were).  The ECL supplier didn't
have any suggestions.  If anyone has come across this problem and hopefully
a solution, I would appreciate any suggestions. 
 
Regards  
James Stiehr



More information about the Bioforum mailing list