splicing

Benoit_Hebert at IAF.UQUEBEC.CA Benoit_Hebert at IAF.UQUEBEC.CA
Mon Apr 10 09:03:41 EST 1995


gisela hoschek (hoschek at JEEVES.UCSD.EDU) wrote:
>  My name Khawaja Sirajuddin. I am a student in Japan. I am from Pakistan.
>  Presently I am doing research in Computer Bio-informatics. Basically I
>  belong to computer field, so I don't know much about Molecular biology.
>  I am working on prediction of splice site junctions in DNA sequences.
>  I would like to know, if there are any other knowledge used in splicing
>  beside consensus sequences, which are they and for that which book is
>  good as reference. I have seen few papers on this topic but without a
>  book it is bit difficult to understand.

You may want to look at the following:

Reviews:
The spliceosome. 1993. BioEssays 15(9) 595-603
Guide to the heart of the spliceosome. 1993. Science 262:1978-1979
Ann. Review of Biochem. 1986 (old...) 55:1119-1150

Primary:
Mutational analysis of py tract: J. Biol. Chem. 1993.  268(15):11222-11229
A survey on intron lenght. 1988. NAR 16(21): 9893-9908
A reappraisal of non-consensus mRNA splice sites. 1991. NAR 19(14) 3795-8
NAR 1994. 22(13) 2525-2531
NAR 1991. 20(10) 2511-2516
NAR 1993. 21(22) 5256-5263
MCB 1991. 11(12) 6075-6083
MCB 1994. 14(1)  437-445
MCB 1993. 13(8)  4939-4952
Gene 1994. 141   171-177

You may want to look a a paper by Zhao et al.:
       J. Virology 1994. 68(5) 2849-2859
In particular, parvoviruses are a good example when it comes to using 
non-consensus splice sites for regulation of splicing. Furthermore, by
looking at the sequences, you will find that (especially for the porcine
parvovirus) combination of poor consensus signals and strong signals make
for efficient splicing.

Good luck,

Benoit Hebert

========================================
Benoit_Hebert at iaf.uquebec.ca

Virology Research Center
Armand-Frappier Institute
Laval, Quebec, Canada
========================================




More information about the Bioforum mailing list