Human Genome Project
mkennedy at chmeds.ac.nz
Sat Apr 15 05:10:28 EST 1995
In article <Pine.SUN.3.91.950412173251.23773B-100000 at cbl.umd.edu>, James Burns <burns at cbl.umd.edu> writes:
> I have participated in many discussions about the human genome project. I
> have answered many questions, but have asked many more. So, here is one
> more for the crowd.
> Whose DNA is being sequenced? It is a very simple question, but a very
> important one.
The simple answer to this question is that BITS of the genomes of
MANY people are being sequenced. Every lab has their own sources
of DNA, and their own reasons for sequencing that particular DNA.
Perhaps they have a particularly good genomic library, made from
one individual, that they source their clones from for
It is fair to say, though, that there will be vastly
different contributions by different genomes to the completed
project. The major YAC libraries used in the genome project,
derived from different individuals (or a cell line from
each individual), are key resources for genome sequencers
and they will represent a disproportionately large part
of the final sequence. The reagents used in the construction of
these YAC libraries (but not the names of the individuals from
which the DNA was derived) will be detailed in the papers
describing their construction. Many other laboratories will
contribute the sequence of one or two genes to the databases, and
these may be derived from the many hundreds of different genomic
libraries in existence.
So, while the completed human genome sequence will be derived
from many hundreds or even thousands of different human genomes,
one or two different individuals may be vastly over-represented
in the final sequence. However, given that (on average) each
human genome has something like 30 million polymorphic bases
the final sequence will only be an approximation, and will not
be a complete representation of any individual genome.
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
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