conjugation in E. coli ?
amuelle3 at gwdu19.gwdg.de
Sat Aug 26 12:32:39 EST 1995
I'm a student of biology in Germany an do some studies in recombination.
I've read in several literature that conjugation in E. coli works as follows:
1. ssDNA-break in donor ...
2. transfer of ssDNA with 5'-end to recipient ...
In an paper (from Sep 1994) I've read that you believe conjugation
occures via dsDNA-break in donor (DNA: circular -> linear ) and transfer with
3'-end at first! The recipient is a covalently closed chromosome that
integrates the donor DNA via recombination.
Hm, which is the way E. coli proceeds conjugation? Is the "up-to-date
recombination-model" a double strand break-model?
If you can help me, please send me an email.
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