Hemoglobin and Cyanide
tivol at news.wadsworth.org
Fri Dec 1 17:29:47 EST 1995
Bob Hoesch (Bob_Hoesch at fws.gov) wrote:
: If one is using hemoglobin as a marker for analyzing population
: genetics, a standard procedure is to treat the blood sample with low
: concentrations of cyanide prior to IEF (isoelectric focusing). This
: results in the elimination of "spurious" bands, and apparently makes
: the resulting banding patterns amenable to genetic analysis. I've heard
: this procedure described as "reduction of methemoglobin" (reduction of
: the oxidized iron back to the reduced state), but this doesn't make sense
: to me in terms of the chemistry. Can someone explain what is happening in
: this cyanide-induced reduction in the number of hemoglobin bands? How could
: cyanide reduce Fe3+ to Fe2+?
Just a thought, but Fe3+ + CN- = Fe(2+)CN.
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