Most Probable Number (MPN). What is the the right to do it?

Flemming Vester fvest at
Tue Dec 19 06:51:42 EST 1995

What is the right way to do an MPN (most probable number)analysis?

There seem to be some confusion in the literature on how the analysis should be
done. I have come acros two different ways:

Some researchers make one single dilution serie, and transfer samples from this
serie into f.ex. three replicates.

Other researchers for example, do three individual dilution series.

As far as I can see, the two used methods are different, and require two
different statistical methods. However, the same tables and computer programs
are applied for calculating MPN's in the litterature.

I hope somebody is able to help me with this problem.

Yours sincerely,

	Flemming Vester
 	Department of Biology
  	Sect. of Microbial Ecology
  	Ny Munkegade, Build. 540 
  	University of Aarhus
  	DK-8000 Aarhus, Denmark

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