antibiotic genes from high GC organisms

Terri Dunahay Terri_Dunahay at NREL.GOV
Fri Feb 24 10:44:31 EST 1995

I am interested in obtaining antibiotic resistance genes, in particular 
neomycin phosphotransferase or neomycin acetyl transferase, from 
microorganisms with DNA with a high GC content.  For example, I know there are 
some clones out there containing aminoglycoside phosphotransferases from 
Streptomyces rimosus and a bleomycin binding protein gene 
from Streptoalloteichus hindustanus.   If there is anyone out there who has 
these (or other) clones who would be willing to send me a plasmid 
or who would like to collaborate, please send me an e-mail message at 
dunahayt at tcplink.nrel gov.  Alternatively, any information on high GC or 
thermophilic strains that produce antibiotic resistance genes would be greatly 

Thanks for your help.

Terri Dunahay 

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