Electrophoresis Gel?
Martin Kennedy
mkennedy at chmeds.ac.nz
Mon Feb 27 05:13:03 EST 1995
In article <3ijn3a$abc at ixnews3.ix.netcom.com>, Fegan1 at ix.netcom.com (Jim Fegan) writes:
> Our teacher is running on a short budget though, and she is
> reluctant about the use of agarose gel because of it's cost. Our lab
> group was looking for a possible alternative to the agarose. Our ideas
> were few, and we even tried geletine, but it ended up melting under the
> current.
> Does anyone know of a gel that would provide a suitable matrix
> for the electrophoresis? If you could email me any suggestions you
> might have, I would greatly appreciate it.
>
> Thank you in advance,
> Jim
Agarose is a purified component of good old-fashioned bacteriological agar,
and I believe agar works quite well for DNA gels (though I've never been
driven to try it). It would certainly be a lot cheaper than agarose. If
you use agarose, and run the gels clean of DNA, you can recycle them -
either as is, or by remelting and pouring them. I wouldn't recommend
either method for serious experiemnts though!
--
Cheers,
Martin
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