ANTIGENICITY OF GLICOPROTEINS (fwd)
haoxiao at cc.UManitoba.CA
Mon Jan 23 23:39:54 EST 1995
Jose Antonio Rodriguez Rodriguez writes:
> Original-To: bioforum at dl.ac.uk
> Many glycoproteins have an antigenic role due to the its glycidic part,
> when we oxidize the glycoproteins with metaperiodate we detect by
> western-blot a band not detected when we don't oxidize glycoproteins.
> Anybody know what is the cause for this? and reference literature about It?
Are you sure? Detected by what, Ab? oxidized glycoprotein should lose
its binding to Ab raised by intact glycoprotein.
If your just want ot detect the sugar moiety of the protein, the oxidized
sugar should form covalently-linked Shiff-base with the enzymes (pH>7).
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