Question about Concanavalin A (Con A) chromatography

Rory.Morty Morty at bchm.unp.ac.za
Wed Jul 5 15:42:01 EST 1995


Hi there!

I have a question about affinity chromatography with Concanavalin A (Con A). 
Once the sample intended for fractionation has been applied and eluted from 
the Con A column, say with methyl mannoside or methyl glucoside, how should 
the column be treated again before the next sample is applied. Do you have 
to get rid of the now tightly boung mannosides and glucosides that were used 
to elute the previous sample? If so, how?

Any information would be appreciated.

Rory Morty
e-mail: morty at gate2.cc.unp.ac.za



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