Chloroplast Suspension Protocol?

[Ben Levin]

Hello all,

 I'm looking for a protocol for separating chloroplasts (centrifugation,
yes?) and keeping them for biochemical analysis, i.e. observing various
environmental (chemical, photo-illumination) effects on fine structure. 
 I plan to begin a search of our library resources tomorrow, but I thought
the extra help I could get from someone out there with experience would be
a great advantage.

Ben
benjinsl at astro.ocis.temple.edu