Using fluorescent dyes for calcium measurements in mitos

[Hans-Georg Klieber]

We woud like to measure the calcium content of mitochondria
in intact heart muscle cells using fluorescent dyes.  

Does anyone have experience with this type of measurement?
Can you give any hints as to the application of the dyes,
specificity of measurement, sensitivity, suitability of
the dyes?

Any advice/recommendation/hint much appreciated.
Ulrike Langheinrich


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Dr. Ulrike Langheinrich
Institute for Physiology, University of Marburg
Deutschhausstrasse 2, 35033 Marburg, Germany
Office: +49-6421-282152